名為FasL (Fas ligand)的跨膜蛋白是在免疫調(diào)控中有重要作用的腫瘤壞死因子家族中的一員。FasL與其受體的結(jié)合誘導(dǎo)細(xì)胞凋亡,，但尚不清楚細(xì)胞死亡對FasL的細(xì)胞功能有多重要,。用剔除相關(guān)基因的突變小鼠（它們?nèi)笔Х置诘腇asL、但卻表達(dá)正常水平的與膜結(jié)合的FasL,；或者缺失與膜結(jié)合的FasL,、但卻仍能產(chǎn)生分泌的FasL）所做實(shí)驗(yàn)表明，可溶FasL通過不涉及細(xì)胞凋亡的機(jī)制促進(jìn)自體免疫和腫瘤形成,。（生物谷bioon.com）
生物谷推薦原文簡介：
Nature 461, 659-663 (1 October 2009) | doi:10.1038/nature08402
Membrane-bound Fas ligand only is essential for Fas-induced apoptosis
Lorraine A. O' Reilly1, Lin Tai1, Lily Lee1, Elizabeth A. Kruse1,2, Stephanie Grabow1,2, W. Douglas Fairlie1, Nicole M. Haynes3, David M. Tarlinton1, Jian-Guo Zhang1, Gabrielle T. Belz1, Mark J. Smyth3, Philippe Bouillet1, Lorraine Robb1 & Andreas Strasser1
1 The Walter and Eliza Hall Institute of Medical Research, Parkville, Victoria 3052, Australia
2 Department of Medical Biology, The University of Melbourne, Parkville, Victoria 3010, Australia
3 Peter MacCallum Cancer Centre, East Melbourne, Victoria 3002, Australia
4 Correspondence to: Andreas Strasser1 Correspondence and requests for materials should be addressed to A.S.
Fas ligand (FasL), an apoptosis-inducing member of the TNF cytokine family, and its receptor Fas are critical for the shutdown of chronic immune responses1, 2, 3 and prevention of autoimmunity4, 5. Accordingly, mutations in their genes cause severe lymphadenopathy and autoimmune disease in mice6, 7 and humans8, 9. FasL function is regulated by deposition in the plasma membrane and metalloprotease-mediated shedding10, 11. Here we generated gene-targeted mice that selectively lack either secreted FasL (sFasL) or membrane-bound FasL (mFasL) to resolve which of these forms is required for cell killing and to explore their hypothesized non-apoptotic activities. Mice lacking sFasL (FasLs/s) appeared normal and their T cells readily killed target cells, whereas T cells lacking mFasL (FasLm/m) could not kill cells through Fas activation. FasLm/m mice developed lymphadenopathy and hyper-gammaglobulinaemia, similar to FasLgld/gld mice, which express a mutant form of FasL that cannot bind Fas, but surprisingly, FasLm/m mice (on a C57BL/6 background) succumbed to systemic lupus erythematosus (SLE)-like autoimmune kidney destruction and histiocytic sarcoma, diseases that occur only rarely and much later in FasLgld/gld mice. These results demonstrate that mFasL is essential for cytotoxic activity and constitutes the guardian against lymphadenopathy, autoimmunity and cancer, whereas excess sFasL appears to promote autoimmunity and tumorigenesis through non-apoptotic activities.
