哈佛大學(xué)醫(yī)學(xué)院、麻省總醫(yī)院等處的科學(xué)家在干細(xì)胞研究發(fā)現(xiàn)取得新的進(jìn)展,，相關(guān)成果發(fā)表在最新一期的Science雜志上,。
哺乳動(dòng)物的心臟是由多種肌肉和非肌肉細(xì)胞所組成的，它們來自2組不同的先祖細(xì)胞,。對(duì)這些先祖細(xì)胞的身份以及導(dǎo)致心室發(fā)育的途徑進(jìn)行精確的界定對(duì)理解心臟是如何發(fā)育及再生心血管藥物的研發(fā)來說都是至關(guān)重要的,。
研究小組在發(fā)現(xiàn)了一個(gè)可產(chǎn)生心室肌肉細(xì)胞的先祖細(xì)胞群。迄今之前,，科研人員無法做到的一件事就是如何誘使細(xì)胞形成假設(shè)能在患病的心臟中行使功能的健康心肌,。
Ibrahim Domian及其同僚應(yīng)用一個(gè)由紅色和綠色熒光標(biāo)簽所組成的系統(tǒng)來標(biāo)記小鼠胚胎發(fā)育中的心臟細(xì)胞組。這一系統(tǒng)使得研究人員能夠分離出專門產(chǎn)生心肌的先祖細(xì)胞,，這些細(xì)胞接著被用來構(gòu)建功能性的"會(huì)搏動(dòng)"的心室肌組織,。 文章的作者說，這種將組織工程與干細(xì)胞生物學(xué)相結(jié)合的方法現(xiàn)在可能使病人的及疾病特異性的心臟先祖細(xì)胞分離出來,。（生物谷Bioon.com）
生物谷推薦：實(shí)驗(yàn)方法
生物谷推薦原始出處：
Science 16 October 2009:DOI: 10.1126/science.1177350
Generation of Functional Ventricular Heart Muscle from Mouse Ventricular Progenitor Cells
Ibrahim J. Domian,1,2,* Murali Chiravuri,1,* Peter van der Meer,1,3,* Adam W. Feinberg,4 Xi Shi,1 Ying Shao,1 Sean M. Wu,1,2 Kevin Kit Parker,2,4,5 Kenneth R. Chien1,2,6,
The mammalian heart is formed from distinct sets of first and second heart field (FHF and SHF, respectively) progenitors. Although multipotent progenitors have previously been shown to give rise to cardiomyocytes, smooth muscle, and endothelial cells, the mechanism governing the generation of large numbers of differentiated progeny remains poorly understood. We have employed a two-colored fluorescent reporter system to isolate FHF and SHF progenitors from developing mouse embryos and embryonic stem cells. Genome-wide profiling of coding and noncoding transcripts revealed distinct molecular signatures of these progenitor populations. We further identify a committed ventricular progenitor cell in the Islet 1 lineage that is capable of limited in vitro expansion, differentiation, and assembly into functional ventricular muscle tissue, representing a combination of tissue engineering and stem cell biology.
1 Cardiovascular Research Center, Massachusetts General Hospital, Charles River Plaza, CPZN 3200, 185 Cambridge Street, Boston, MA 02114–2790, USA.
2 Harvard Stem Cell Institute, Cambridge, MA 02138, USA.
3 Department of Cardiology, University Medical Center Groningen, Hanzeplein 1, 9700 RB Groningen, Netherlands.
4 Disease Biophysics Group, School of Engineering and Applied Sciences, Harvard University, Cambridge, MA 02138, USA.
5 The Wyss Institue for Biologically Inspired Engineering, Harvard University, Boston, MA 02115, USA.
6 Department of Stem Cell and Regenerative Biology, Harvard University, Cambridge, MA 02138, USA.
