當(dāng)期Cell Stem Cell封面：一個(gè)橙子慢慢變成蘋果，象征著細(xì)胞重編程過程
誘導(dǎo)多能性干細(xì)胞（induced pluripotent stem cells,，iPS cells）是具有胚胎干細(xì)胞特性的一種細(xì)胞,，由體細(xì)胞誘導(dǎo)而來，可作為胚胎干細(xì)胞最好的替代品用于科學(xué)研究和臨床治療,。但是iPS細(xì)胞的成功誘導(dǎo)比較復(fù)雜,，誘導(dǎo)效率一直是科學(xué)界的難題，通常每萬個(gè)細(xì)胞才能誘導(dǎo)成功一例iPS細(xì)胞,。
最近,，中科院廣州生物醫(yī)藥與健康研究院裴端卿帶領(lǐng)的研究小組發(fā)現(xiàn)，通過在培養(yǎng)過程中添加維生素C可使iPS誘導(dǎo)效率提高10倍,。通過老鼠和人細(xì)胞實(shí)驗(yàn)發(fā)現(xiàn),，培養(yǎng)時(shí)添加維生素C可促進(jìn)相關(guān)基因表達(dá)，推動(dòng)體細(xì)胞進(jìn)入重編程狀態(tài),。
該項(xiàng)成果將于美國時(shí)間今年12月24日在世界干細(xì)胞權(quán)威雜志《細(xì)胞—干細(xì)胞》（Cell Stem Cell）在線發(fā)布,，并且被選為封面文章。（生物谷Bioon.com）
提高iPS誘導(dǎo)效率方式：
Nature：iPS細(xì)胞轉(zhuǎn)化率提高約百倍
Nature Methods：新方法可提高成體組織轉(zhuǎn)化iPS細(xì)胞效率
Nature：高效誘導(dǎo)iPS的方法模型
Cell Stem Cell：兩種因子可提高iPS轉(zhuǎn)化率
Nature Biotechnology：新方法大幅提高IPS生產(chǎn)率
Cell Stem Cell：低氧環(huán)境可提高培養(yǎng)iPS細(xì)胞效率
各種誘導(dǎo)iPS方法：
Cell Stem Cell：利用重組蛋白誘導(dǎo)iPS細(xì)胞成功
Cloning and Stem Cells：通過控制培養(yǎng)條件可激發(fā)體細(xì)胞的干細(xì)胞特性
Science：不含外來DNA的人類iPS細(xì)胞
Nature：不使用病毒載體生成人iPS細(xì)胞系的方法
Cell Stem Cell：化學(xué)小分子可替代基因誘導(dǎo)iPS細(xì)胞
Cell：利用單基因調(diào)控誘導(dǎo)iPS
生物谷推薦原始出處：
Cell Stem Cell, 24 December 2009 doi:10.1016/j.stem.2009.12.001
Vitamin C Enhances the Generation of Mouse and Human Induced Pluripotent Stem Cells
Miguel Angel Esteban1, 6, Tao Wang1, 6, Baoming Qin1, 6, Jiayin Yang1, Dajiang Qin1, Jinglei Cai1, Wen Li1, Zhihui Weng1, Jiekai Chen1, Su Ni1, Keshi Chen1, Yuan Li1, Xiaopeng Liu1, Jianyong Xu1, Shiqiang Zhang1, Feng Li1, Wenzhi He1, Krystyna Labuda2, Yancheng Song3, Anja Peterbauer4, Susanne Wolbank2, Heinz Redl2, Mei Zhong5, Daozhang Cai3, Lingwen Zeng1 and Duanqing Pei1, ,
1 Stem Cell and Cancer Biology Group, Key Laboratory of Regenerative Biology, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510663, China
2 Ludwig Boltzmann Institute for Clinical and Experimental Traumatology, Austrian Cluster for Tissue Regeneration, Vienna 1200, Austria
3 The Third Affiliated Hospital of Sun Yat-Sen University, Guangzhou 510630, China
4 Red Cross Blood Transfusion Service of Upper Austria, Austrian Cluster for Tissue Regeneration, Linz 4020, Austria
5 Center for Prenatal and Hereditary Disease Diagnosis, Nanfang Hospital, Guangzhou 510515, China
Somatic cells can be reprogrammed into induced pluripotent stem cells (iPSCs) by defined factors. However, the low efficiency and slow kinetics of the reprogramming process have hampered progress with this technology. Here we report that a natural compound, vitamin C (Vc), enhances iPSC generation from both mouse and human somatic cells. Vc acts at least in part by alleviating cell senescence, a recently identified roadblock for reprogramming. In addition, Vc accelerates gene expression changes and promotes the transition of pre-iPSC colonies to a fully reprogrammed state. Our results therefore highlight a straightforward method for improving the speed and efficiency of iPSC generation and provide additional insights into the mechanistic basis of the reprogramming process.? Vitamin C improves the speed and efficiency of mouse iPSC generation . Adding vitamin C converts pre-iPSCs to iPSCs . Vitamin C alleviates the senescence roadblock to reprogramming . Human iPSC generation is also improved by vitamin C
