日本慶應(yīng)義塾大學(xué)教授福田惠一和助教下地顯一郎近日發(fā)現(xiàn),，一種名為“粒細(xì)胞集落刺激因子”的物質(zhì)可以幫助心肌細(xì)胞大量增殖,。
老鼠胎兒在發(fā)育初期，心肌細(xì)胞會(huì)迅速增殖,。福田對(duì)在母鼠子宮中發(fā)育了10天的老鼠胎兒進(jìn)行了專門(mén)研究,。他發(fā)現(xiàn)，這一時(shí)期老鼠胎兒體內(nèi)的“粒細(xì)胞集落刺激因子”數(shù)量增加,，于是猜測(cè)這種因子與心肌細(xì)胞增殖相關(guān),。
為了證明這一猜測(cè)，福田利用胚胎干細(xì)胞技術(shù)培育出了猴子的心肌細(xì)胞,，然后加入“粒細(xì)胞集落刺激因子”,，結(jié)果猴子心肌細(xì)胞的數(shù)量增加了數(shù)十倍。這一成果發(fā)表在最新一期美國(guó)《細(xì)胞—干細(xì)胞》（Cell Stem Cell）雜志上,。
此前,，科學(xué)家已經(jīng)發(fā)現(xiàn)，“粒細(xì)胞集落刺激因子”可以刺激骨髓產(chǎn)生大量白細(xì)胞,，用于治療由化療導(dǎo)致的白細(xì)胞減少,。福田指出，這次的成果可能會(huì)把“粒細(xì)胞集落刺激因子”進(jìn)一步推向再生醫(yī)療的前臺(tái),。（生物谷Bioon.com）
生物谷推薦原始出處：
Cell Stem Cell, Volume 6, Issue 3, 227-237, 5 March 2010 DOI:10.1016/j.stem.2010.01.002
G-CSF Promotes the Proliferation of Developing Cardiomyocytes In Vivo and in Derivation from ESCs and iPSCs
Kenichiro Shimoji, Shinsuke Yuasa, Takeshi Onizuka, Fumiyuki Hattori, Tomofumi Tanaka, Mie Hara, Yohei Ohno, Hao Chen, Toru Egasgira, Tomohisa Seki, Kojiro Yae, Uichi Koshimizu, Satoshi Ogawa, Keiichi Fukuda
Department of Regenerative Medicine and Advanced Cardiac Therapeutics, Keio University School of Medicine, Tokyo 160-8582, Japan Cardiology Division, Department of Internal Medicine, Keio University School of Medicine, Tokyo 160-8582, Japan Biomedical Research Laboratories, Asubio Pharma Co., Ltd., Tokyo 618-8513, Japan Corresponding author These authors contributed equally to this work
During a screen for humoral factors that promote cardiomyocyte differentiation from embryonic stem cells (ESCs), we found marked elevation of granulocyte colony-stimulating factor receptor (G-CSFR) mRNA in developing cardiomyocytes. We confirmed that both G-CSFR and G-CSF were specifically expressed in embryonic mouse heart at the midgestational stage, and expression levels were maintained throughout embryogenesis. Intrauterine G-CSF administration induced embryonic cardiomyocyte proliferation and caused hyperplasia. In contrast, approximately 50% of csf3r–/– mice died during late embryogenesis because of the thinning of atrioventricular walls. ESC-derived developing cardiomyocytes also strongly expressed G-CSFR. When extrinsic G-CSF was administered to the ESC- and human iPSC-derived cardiomyocytes, it markedly augmented their proliferation. Moreover, G-CSF-neutralizing antibody inhibited their proliferation. These findings indicated that G-CSF is critically involved in cardiomyocyte proliferation during development, and may be used to boost the yield of cardiomyocytes from ESCs for their potential application to regenerative medicine.
