2009年末,，3D電影阿凡達(dá)風(fēng)靡全球,，細(xì)胞的世界其實(shí)也有3D的精彩,，一個(gè)來自Houston醫(yī)學(xué)研究中心的研究團(tuán)隊(duì)開發(fā)出一種3D細(xì)胞培養(yǎng)技術(shù),，相關(guān)成果文章公布在最新一期的Nature Nanotechnology上,。
相比阿凡達(dá)的3D技術(shù)，在實(shí)驗(yàn)室用3D技術(shù)培養(yǎng)細(xì)胞簡(jiǎn)單得多,，只要在培養(yǎng)皿中提供一個(gè)磁力,，使得細(xì)胞懸浮在培養(yǎng)皿中就可以輕松獲得，這樣細(xì)胞在培養(yǎng)皿中的環(huán)境更像在組織中生長(zhǎng)的環(huán)境,。
傳統(tǒng)細(xì)胞培養(yǎng)技術(shù)在模擬細(xì)胞體內(nèi)生存環(huán)境方面做得還不夠,。3D細(xì)胞培養(yǎng)技術(shù)的發(fā)明就是為了在細(xì)胞培養(yǎng)過程中，為細(xì)胞提供一個(gè)更加接近體內(nèi)生存條件的微環(huán)境,。
文章的作者之一Tom Killian副教授表示,，用3D細(xì)胞培養(yǎng)技術(shù)可使得細(xì)胞狀態(tài)保持與在人體內(nèi)生長(zhǎng)的相似，這對(duì)藥物篩選等研究具有重要的意義,。如果說,，我們可以提高藥物篩選的可信度，只要提高10%,，就能節(jié)省1億美金的研發(fā)經(jīng)費(fèi),。
德克薩斯大學(xué)Anderson癌癥中心的David H.Koch表示，在癌癥研究中,，3D技術(shù)顯得更為關(guān)鍵,，我們下一步的計(jì)劃是將這些3D技術(shù)用于癌癥方面的研究。
3D細(xì)胞培養(yǎng)技術(shù)為細(xì)胞提供一個(gè)更加接近體內(nèi)生存條件的微環(huán)境,，感覺就像“在家里一樣”,。該技術(shù)不僅僅應(yīng)用于基于細(xì)胞的高通量藥物篩選，還可以應(yīng)用于醫(yī)學(xué)中,。
3D細(xì)胞培養(yǎng),，細(xì)胞生長(zhǎng)更接近體內(nèi)環(huán)境——感覺像“在家里一樣”。因此,，研究者通過3D培養(yǎng)技術(shù)從高通量篩選,、毒物篩選及其它篩選中來獲得接近體內(nèi)真實(shí)情況的數(shù)據(jù)。(生物谷Bioon.com)
生物谷推薦原文出處：
Nature Nanotechnology doi:10.1038/nnano.2010.23
Three-dimensional tissue culture based on magnetic cell levitation
Glauco R. Souza1,9, Jennifer R. Molina2, Robert M. Raphael3, Michael G. Ozawa1, Daniel J. Stark4, Carly S. Levin5, Lawrence F. Bronk1, Jeyarama S. Ananta6, Jami Mandelin1, Maria-Magdalena Georgescu2, James A. Bankson7, Juri G. Gelovani8, T. C. Killian4, Wadih Arap1 & Renata Pasqualini1
Cell culture is an essential tool in drug discovery, tissue engineering and stem cell research. Conventional tissue culture produces two-dimensional cell growth with gene expression, signalling and morphology that can be different from those found in vivo, and this compromises its clinical relevance1, 2, 3, 4, 5. Here, we report a three-dimensional tissue culture based on magnetic levitation of cells in the presence of a hydrogel consisting of gold, magnetic iron oxide nanoparticles and filamentous bacteriophage. By spatially controlling the magnetic field, the geometry of the cell mass can be manipulated, and multicellular clustering of different cell types in co-culture can be achieved. Magnetically levitated human glioblastoma cells showed similar protein expression profiles to those observed in human tumour xenografts. Taken together, these results indicate that levitated three-dimensional culture with magnetized phage-based hydrogels more closely recapitulates in vivo protein expression and may be more feasible for long-term multicellular studies.
