植物學(xué)國(guó)際頂級(jí)刊物Plant Physiology（IF=6.982 2009年度影響因子十大看點(diǎn)）7月1日最新一期發(fā)表了浙江大學(xué)果實(shí)品質(zhì)生物學(xué)研究團(tuán)隊(duì)2010屆博士生殷學(xué)仁題為《獼猴桃AdEIL和AdERF基因參與調(diào)控果實(shí)成熟相關(guān)基因表達(dá)》的論文。
乙烯是重要植物激素,，參與植物整個(gè)生命進(jìn)程,，也是影響果實(shí)成熟衰老最重要的植物激素之一。乙烯生物學(xué)研究包括乙烯生物合成和乙烯信號(hào)轉(zhuǎn)導(dǎo)兩大主題,，長(zhǎng)期以來(lái)植物學(xué)界存在一科學(xué)猜想——乙烯信號(hào)轉(zhuǎn)導(dǎo)能否反饋調(diào)控乙烯生物合成,。
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該論文從獼猴桃果實(shí)乙烯信號(hào)轉(zhuǎn)導(dǎo)途徑兩個(gè)轉(zhuǎn)錄因子基因家族（EIN3/EIL和ERF）入手,，發(fā)現(xiàn)獼猴桃果實(shí)AdEIL2和AdEIL3可活化乙烯合成關(guān)鍵基因AdACO1啟動(dòng)子,，進(jìn)而調(diào)控乙烯合成相關(guān)基因轉(zhuǎn)錄,，并加速植株乙烯合成，首次系統(tǒng)的闡述了EIN3/EILs可作為乙烯信號(hào)轉(zhuǎn)導(dǎo)反饋調(diào)控乙烯生物合成的關(guān)鍵位點(diǎn),，該研究結(jié)果為果實(shí)乙烯生物合成調(diào)控提供了新的理論依據(jù),。(生物谷Bioon.net)
生物谷推薦原文出處：
Plant Physiology DOI:10.1104/pp.110.157081
Kiwifruit EIL and ERF genes involved in regulating fruit ripening
Xue-ren Yin1, Andrew C. Allan2, Kun-song Chen1,3 and Ian B. Ferguson2
1 Zhejiang University; , 2 New Zealand Institute for Plant and Food Research Limited
Kiwifruit (Actinidia deliciosa) is a climacteric fruit sensitive to low concentrations of ethylene. To investigate the transcriptional mechanisms underlying kiwifruit ethylene response, transcription factors encoding four EIN3-Like (EILs) and fourteen ethylene response factors (ERFs) were cloned from kiwifruit. Expression of these transcription factors was examined during fruit development. The expression of transcripts of most AdERFs was higher during early fruit development, with the exception of AdERF3 which increased with maturity. Several AdERFs were apparently downregulated by ethylene as they were affected by the ethylene inhibitor 1-methylcyclopropene and by antisense suppression of ACO in the fruit. In contrast, AdEILs were constitutively expressed during fruit development and ripening. The transcription factors AdEIL2 and AdEIL3 activated transcription of the ripening-related genes AdACO1 and AdXET5 and, when over-expressed in Arabidopsis, stimulated ethylene production. The potential repressor AdERF9 suppressed this promoter activity. These results support a role for kiwifruit EILs and ERFs in transcriptional regulation of ripening related genes and in the regulation of kiwifruit fruit ripening processes.
