肌漿球蛋白家族的運(yùn)動(dòng)蛋白在真核細(xì)胞中有很多重要功能,，而且是基于肌動(dòng)蛋白的運(yùn)動(dòng)的關(guān)鍵,。我們對(duì)肌漿球蛋白作用機(jī)制的認(rèn)識(shí)一直受阻于缺乏使我們能夠?qū)ι锓肿拥慕Y(jié)構(gòu)和動(dòng)態(tài)同時(shí)進(jìn)行觀察的技術(shù)，但現(xiàn)在Kodera等人利用高速原子力顯微鏡(HS-AFM) 以前所未有的時(shí)間分辨率對(duì)沿肌動(dòng)蛋白細(xì)絲運(yùn)動(dòng)的肌漿球蛋白-V直接進(jìn)行了觀察,。他們獲得了高分辨率的影片,，這些影片提供了支持有關(guān)肌漿球蛋白運(yùn)動(dòng)的“擺動(dòng)杠桿臂”模型的視覺證據(jù)。
利用HS-AFM進(jìn)行高分辨率成像成為可能,，將使該方法能廣泛適用于結(jié)構(gòu)生物學(xué)和單分子生物學(xué)領(lǐng)域,。（生物谷Bioon.com）
生物谷推薦英文摘要：
Nature doi:10.1038/nature09450
Video imaging of walking myosin V by high-speed atomic force microscopy
Noriyuki Kodera,Daisuke Yamamoto,Ryoki Ishikawa& Toshio
The dynamic behaviour of myosin V molecules translocating along actin filaments has been mainly studied by optical microscopy. The processive hand-over-hand movement coupled with hydrolysis of adenosine triphosphate was thereby demonstrated. However, the protein molecules themselves are invisible in the observations and have therefore been visualized by electron microscopy in the stationary states. The concomitant assessment of structure and dynamics has been unfeasible, a situation prevailing throughout biological research. Here we directly visualize myosin V molecules walking along actin tracks, using high-speed atomic force microscopy. The high-resolution movies not only provide corroborative ‘visual evidence’ for previously speculated or demonstrated molecular behaviours, including lever-arm swing, but also reveal more detailed behaviours of the molecules, leading to a comprehensive understanding of the motor mechanism. Our direct and dynamic high-resolution visualization is a powerful new approach to studying the structure and dynamics of biomolecules in action.
