研究人員研制出了一種簡化的,、化學(xué)成分明確的培養(yǎng)系統(tǒng)，可用于人類多能干細胞的生長,，新成果發(fā)表在日前在線出版的《自然—方法學(xué)》期刊上,。新方法很重要， 既可用于標準化來自不同實驗室的報告結(jié)果,，也可最終用于這些細胞的臨床應(yīng)用,。
多能干細胞的研究部分屬于科學(xué)，更多屬于藝術(shù),，因為它們對培養(yǎng)環(huán)境有高度的敏感性：如果沒有被恰當(dāng)處置,，它們或者會死亡或者失去其“干細胞性”。過去,，科學(xué)家們的許多工作是努力定義出讓干細胞生長的混合培養(yǎng)液的相關(guān)成分,。
James Thomson和同事報告了對一種干細胞培養(yǎng)液的系統(tǒng)剖析，定義出它的基本成分,。在一項辛苦的工作中,，他們挑選出液體培養(yǎng)液中的8種關(guān)鍵成分，并在化學(xué)上定義出這些成分,。他們還定義出表面涂層的成分,，指出這些成分既能讓培養(yǎng)液長時間高效地培養(yǎng)人類胚胎干細胞，也能長時間高效地培養(yǎng)來自新鮮活組織切片的人類誘導(dǎo)多能干細胞,。（生物谷Bioon.com）
生物谷推薦原文 出處：
Nature Methods DOI:10.1038/nmeth.1593
Chemically defined conditions for human iPSC derivation and culture
Guokai Chen; Daniel R Gulbranson; Zhonggang Hou; Jennifer M Bolin; Victor Ruotti; Mitchell D Probasco; Kimberly Smuga-Otto; Sara E Howden; Nicole R Diol; Nicholas E Propson; Ryan Wagner; Garrett O Lee; Jessica Antosiewicz-Bourget; Joyce M C Teng; James A Thomson
We re-examine the individual components for human embryonic stem cell (ESC) and induced pluripotent stem cell (iPSC) culture and formulate a cell culture system in which all protein reagents for liquid media, attachment surfaces and splitting are chemically defined. A major improvement is the lack of a serum albumin component, as variations in either animal- or human-sourced albumin batches have previously plagued human ESC and iPSC culture with inconsistencies. Using this new medium (E8) and vitronectin-coated surfaces, we demonstrate improved derivation efficiencies of vector-free human iPSCs with an episomal approach. This simplified E8 medium should facilitate both the research use and clinical applications of human ESCs and iPSCs and their derivatives, and should be applicable to other reprogramming methods.
