日本研究人員報(bào)告說(shuō),，他們成功地在體外將實(shí)驗(yàn)鼠的精原干細(xì)胞培育成了精子,，這一成果將有助于男性不育癥的研究,。
日本橫濱市立大學(xué)教授小川毅彥與理化學(xué)研究所合作，從出生8天的雄性實(shí)驗(yàn)鼠體內(nèi)提取出精原干細(xì)胞，并從出生5天至8天的實(shí)驗(yàn)鼠體內(nèi)摘取精巢,，然后將精原干細(xì)胞注入精巢的生精小管內(nèi),，經(jīng)過(guò)43天的培養(yǎng)，精原干細(xì)胞發(fā)育成了精子細(xì)胞,。
研究人員利用這些精子細(xì)胞,，通過(guò)體外受精培育出52個(gè)受精卵，然后植入雌性實(shí)驗(yàn)鼠體內(nèi),，最終產(chǎn)下5只小鼠,。這5只小鼠都健康成長(zhǎng)，其中3只雌鼠后來(lái)通過(guò)自然交配,，又產(chǎn)下了21只小鼠,。研究小組由此認(rèn)為利用這種方法培育的精子具有正常功能。
研究人員還利用精原干細(xì)胞正常,、但精巢內(nèi)其他細(xì)胞異常以致無(wú)法產(chǎn)生精子的不育實(shí)驗(yàn)鼠進(jìn)行了相同實(shí)驗(yàn),，將它們的精原干細(xì)胞注入正常實(shí)驗(yàn)鼠的精巢生精小管培養(yǎng)，也生成了正常精子,。研究小組據(jù)此推測(cè)，一些男性不育癥患者的精原干細(xì)胞也有可能是正常的,。
這一成果發(fā)表在新一期英國(guó)《自然·通信》網(wǎng)絡(luò)雜志上,。小川毅彥說(shuō)，雖然這項(xiàng)技術(shù)目前還無(wú)法直接用于人類,，但通過(guò)觀察試管中精子在精巢內(nèi)形成的過(guò)程,，可以弄清精子形成的必要條件，從而有助于對(duì)男性不育癥的研究,。(生物谷Bioon.com)
doi:10.1038/ncomms1478
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In vitro production of fertile sperm from murine spermatogonial stem cell lines
Takuya Sato,Kumiko Katagiri,Tetsuhiro Yokonishi,Yoshinobu Kubota,Kimiko Inoue,Narumi Ogonuki,Shogo Matoba,Atsuo Ogura & Takehiko Ogawa
Spermatogonial stem cells (SSCs) are the only stem cells in the body that transmit genetic information to the next generation. The long-term propagation of rodent SSCs is now possible in vitro, and their genetic modification is feasible. However, their differentiation into sperm is possible only under in vivo conditions. Here we show a new in vitro system that can induce full spermatogenesis from SSC lines or any isolated SSCs. The method depends on an organ culture system onto which SSCs are transplanted. The settled SSCs form colonies and differentiate up into sperm. The resultant haploid cells are fertile, and give rise to healthy offspring through micro-insemination. In addition, the system can induce spermatogenesis from SSCs that show spermatogenic failure due to a micro-environmental defect in their original testes. Thus, an in vitro system is established that can be used to correct or manipulate the micro-environmental conditions required for proper spermatogenesis from murine SSC lines.
