Cloning and Characterization of TOM7-like Gene in Wheat
張政值,，馬正強(qiáng)
南京農(nóng)業(yè)大學(xué)作物遺傳與種質(zhì)創(chuàng)新國家重點(diǎn)實(shí)驗(yàn)室，南京 210095
Abstract
通過RT-PCR技術(shù)分離了編碼小麥線粒體蛋白轉(zhuǎn)運(yùn)酶TOM7亞基（Translocase of Outer Mitochondrial Membrane subunit 7）的cDNA,，并克隆了該基因編碼區(qū)的基因組DNA,，將該基因命名為TaTOM7,。TaTOM7是一個沒有內(nèi)含子的基因,，其編碼的多肽具有一個位于中部的疏水跨膜區(qū)和兩個分別位于N-端和C-端的親水區(qū)域。來源于真菌,、動物和植物的TOM7類似蛋白在疏水跨膜區(qū)高度保守,，而在親水區(qū)變異很大。在系統(tǒng)發(fā)育樹中,，TOM7類似蛋白可以按動物,、植物和真菌分為3個類群。小麥TaTOM7在基因組中以寡拷貝形式存在,，它的表達(dá)在Ms2近等基因系之間和不同組織之間存在差異,。中國春缺體－四體及端二體系分析表明，TaTOM7位于小麥第3染色體同源群,。
Abstract
The cDNA encoding TOM7 (Translocase of Outer Mitochondrial Membrane subunit 7) like protein in wheat was cloned through RT-PCR, and its genomic DNA fragment was subsequently cloned. This gene was tentatively designated as TaTOM7. It has no intron in the coding region and its product possesses one hydrophobic trans-membrane domain in the middle, and one hydrophilic domain in the N-terminal and C-terminal domain, respectively. The amino acid composition in the trans-membrane domain of the TaTOM7 subunit is highly conserved among plants, animals and fungi. According to the phylogenetic tree, TOM7-like proteins from different species can be classified into three groups, representing plants, animals and fungi respectively. TaTOM7 is a single- or oligo-copy gene in the wheat genome, displaying different expression levels between Ms2 near-isogenic lines in some tissues, suggesting a role of Ms2 in its expression. TaTOM7 was mapped to chromosome group three.
該文發(fā)表在：遺傳學(xué)報(bào) 2005.2 Vol 32, No.2 170-177
