細菌可以通過結(jié)合作用獲得抗性，致病性和改變代謝途徑,。通過應(yīng)用熒光蛋白融合技術(shù)SeqA-YFP,，這一過程可在大腸桿菌中實時展現(xiàn)出來。性菌毛作為DNA媒介使之可在細胞間傳遞,，大約96%的DNA可在受體菌中重組,；其余的在細胞中被核酸酶降解,。獲得的完整DNA通過連續(xù)復(fù)制留下足跡，偶然情況會斷裂或被染色體分隔開來,，所以,，不同的基因可以在細胞中復(fù)制遺傳。這一技術(shù)使基因橫向轉(zhuǎn)移全過程展現(xiàn)在我們面前,。(生物谷）
生物谷推薦原始出處：
Science 14 March 2008:
Vol. 319. no. 5869, pp. 1533 - 1536
DOI: 10.1126/science.1153498
Direct Visualization of Horizontal Gene Transfer
Ana Babi,1,2* Ariel B. Lindner,1,2 Marin Vuli,1,2 Eric J. Stewart,1,2 Miroslav Radman1,2,3
Conjugation allows bacteria to acquire genes for antibiotic resistance, novel virulence attributes, and alternative metabolic pathways. Using a fluorescent protein fusion, SeqA-YFP, we have visualized this process in real time and in single cells of Escherichia coli. We found that the F pilus mediates DNA transfer at considerable cell-to-cell distances. Integration of transferred DNA by recombination occurred in up to 96% of recipients; in the remaining cells, the transferred DNA was fully degraded by the RecBCD helicase/nuclease. The acquired integrated DNA was tracked through successive replication rounds and was found to occasionally split and segregate with different chromosomes, leading to the inheritance of different gene clusters within the cell lineage. The incidence of DNA splitting corresponds to about one crossover per cell generation.
1 INSERM U571, Paris F-75015, France.
2 Université Paris Descartes Faculté de Médecine, Paris F-75015, France.
3 Mediterranean Institute for Life Sciences, Metrovievo etalite bb, 21000 Split, Croatia.
