英國(guó)化學(xué)家13日宣布,，他們開(kāi)發(fā)出一個(gè)實(shí)驗(yàn)?zāi)Ｐ?，可展?0億年前地球生命如何以一種自我復(fù)制的分子形式起源。研究人員在早期地球環(huán)境模擬條件下首次合成了一種中間物質(zhì),，該物質(zhì)可純化合成RNA所需的核糖和堿基,，并最終形成一種類(lèi)RNA。相關(guān)研究發(fā)表在當(dāng)日出版的《自然》雜志上,。
眾所周知,，脫氧核糖核酸（DNA）和核糖核酸（RNA）是生物體的遺傳分子，都由堿基,、核糖和磷酸組成,。DNA具有雙螺旋的復(fù)雜結(jié)構(gòu),，而單鏈的RNA在結(jié)構(gòu)上更為簡(jiǎn)單和牢固。因此有學(xué)者認(rèn)為,，DNA過(guò)于復(fù)雜,，不可能在瞬間突然出現(xiàn)。在生命最初產(chǎn)生時(shí),，DNA的單鏈“表親”———RNA要先于DNA出現(xiàn),，是地球上最早出現(xiàn)的攜帶遺傳信息的核酸。
不過(guò),，這種“RNA第一”理論,，在現(xiàn)實(shí)論證中卻遇到不少問(wèn)題。
RNA包括三個(gè)主要組成部分：堿基,、核糖和磷酸,。在傳統(tǒng)思路看來(lái)，這三種物質(zhì)必須分別形成后再以分子的形式相結(jié)合才能形成RNA,。因此與DNA相比,，RNA雖然更為簡(jiǎn)單，但仍是一個(gè)復(fù)雜分子,，不大可能自行組裝成功,。此前試圖通過(guò)化學(xué)方式來(lái)證明這三種化學(xué)物質(zhì)可以同時(shí)產(chǎn)生的努力也均以失敗告終。
但英國(guó)曼徹斯特大學(xué)化學(xué)家給出了不同解釋,。由約翰·薩瑟蘭教授領(lǐng)導(dǎo)的研究小組大膽提出,，通過(guò)模擬地球早期環(huán)境中的一系列化學(xué)反應(yīng)，可以合成一種重要的中間物質(zhì),，進(jìn)而合成類(lèi)RNA,。
實(shí)驗(yàn)?zāi)Ｐ捅砻鳎瑥?0億年前就已在地球上存在的簡(jiǎn)單化學(xué)物質(zhì)中形成RNA的所有成分是完全可能的,，他們已合成了RNA的三個(gè)組成部分中的兩個(gè),，并首次成功合成出一種類(lèi)RNA。試驗(yàn)先由一種被稱(chēng)為羥基乙醛的簡(jiǎn)單糖類(lèi)開(kāi)始,，而后將其與一種氰化物和氨的合成物———單氫胺以及磷酸鹽發(fā)生反應(yīng),，從而產(chǎn)生出一種被稱(chēng)為2-氨基噁唑的中間物質(zhì)。研究人員發(fā)現(xiàn),，自然界的晝夜溫差可幫助純化2-氨基噁唑,，將其變成充裕的前體，這有助于形成新核苷酸分子的糖和堿基蛋白,。磷酸鹽的存在和來(lái)自太陽(yáng)的紫外線則促成了合成,。
美國(guó)分子生物學(xué)家杰克?索斯塔克在《自然》雜志的相關(guān)評(píng)論中，對(duì)該研究給予了高度評(píng)價(jià),稱(chēng)這將是多年來(lái)通過(guò)化學(xué)方式解釋生命起源研究的一大進(jìn)展，代表了多年來(lái)“前生物化學(xué)”研究所取得的重大進(jìn)展,，前生物化學(xué)是研究導(dǎo)致地球生物出現(xiàn)的化學(xué)進(jìn)程的術(shù)語(yǔ),。（生物谷Bioon.com）
生物谷推薦原始出處：
Nature 459, 239-242 (14 May 2009) | doi:10.1038/nature08013
Synthesis of activated pyrimidine ribonucleotides in prebiotically plausible conditions
Matthew W. Powner1, Béatrice Gerland1 & John D. Sutherland1
1 School of Chemistry, The University of Manchester, Oxford Road, Manchester M13 9PL, UK
At some stage in the origin of life, an informational polymer must have arisen by purely chemical means. According to one version of the 'RNA world' hypothesis1, 2, 3 this polymer was RNA, but attempts to provide experimental support for this have failed4, 5. In particular, although there has been some success demonstrating that 'activated' ribonucleotides can polymerize to form RNA6, 7, it is far from obvious how such ribonucleotides could have formed from their constituent parts (ribose and nucleobases). Ribose is difficult to form selectively8, 9, and the addition of nucleobases to ribose is inefficient in the case of purines10 and does not occur at all in the case of the canonical pyrimidines11. Here we show that activated pyrimidine ribonucleotides can be formed in a short sequence that bypasses free ribose and the nucleobases, and instead proceeds through arabinose amino-oxazoline and anhydronucleoside intermediates. The starting materials for the synthesis—cyanamide, cyanoacetylene, glycolaldehyde, glyceraldehyde and inorganic phosphate—are plausible prebiotic feedstock molecules12, 13, 14, 15, and the conditions of the synthesis are consistent with potential early-Earth geochemical models. Although inorganic phosphate is only incorporated into the nucleotides at a late stage of the sequence, its presence from the start is essential as it controls three reactions in the earlier stages by acting as a general acid/base catalyst, a nucleophilic catalyst, a pH buffer and a chemical buffer. For prebiotic reaction sequences, our results highlight the importance of working with mixed chemical systems in which reactants for a particular reaction step can also control other steps.
