美國農(nóng)業(yè)部農(nóng)業(yè)研究局Roger Thilmony領(lǐng)導(dǎo)的作物改良與利用研究小組近日發(fā)現(xiàn)一種組織特異性啟動(dòng)子LP2,，其屬于光合作用組織中的受體基因，在轉(zhuǎn)基因水稻的葉中高度活躍，在根,、種子和花中未被檢測(cè)到,。
利用LP2啟動(dòng)子促使抗稻瘟病和枯紋病的基因表達(dá),，可以改良水稻,、大麥和小麥的品種，還能在不影響其他組織的情況下,，只通過控制葉的性狀開發(fā)生物能源作物,。（生物谷Bioon.com）
生物谷推薦英文摘要：
Plant Biotechnology Journal DOI: 10.1111/j.1467-7652.2009.00449.x
The LP2 leucine-rich repeat receptor kinase gene promoter directs organ-specific, light-responsive expression in transgenic rice
Roger Thilmony, Mara Guttman, James G. Thomson, Ann E. Blechl
Biotechnologists seeking to limit gene expression to nonseed tissues of genetically engineered cereal crops have only a few choices of well characterized organ-specific promoters. We have isolated and characterized the promoter of the rice Leaf Panicle 2 gene (LP2, Os02g40240). The LP2 gene encodes a leucine-rich repeat-receptor kinase-like protein that is strongly expressed in leaves and other photosynthetic tissues. Transgenic rice plants containing an LP2 promoter-GUS::GFP bifunctional reporter gene displayed an organ-specific pattern of expression. This expression corresponded to transcript levels observed on RNA blots of various rice organs and microarray gene expression data. The strongest β-glucuronidase activity was observed in histochemically stained mesophyll cells, but other green tissues and leaf cell types including epidermal cells also exhibited expression. Low or undetectable levels of LP2 transcript and LP2-mediated reporter gene expression were observed in roots, mature seeds, and reproductive tissues. The LP2 promoter is highly responsive to light and only weak expression was detected in etiolated rice seedlings. The specificity and strength of the LP2 promoter suggests that this promoter will be a useful control element for green tissue-specific expression in rice and potentially other plants. Organ-specific promoters like LP2 will enable precise, localized expression of transgenes in biotechnology-derived crops and limit the potential of unintended impacts on plant physiology and the environment.
