關(guān)鍵詞： 分析儀器銷售排行
生物谷報(bào)道：New England Biolabs的華人科學(xué)家Huimin Kong及其同事已經(jīng)成功研制出一種新的DNA擴(kuò)增方法,，這種方法有望取代現(xiàn)有的PCR擴(kuò)增DNA技術(shù)——PCR技術(shù)對(duì)醫(yī)學(xué)診斷和基礎(chǔ)研究的意義重大，但卻只能局限在實(shí)驗(yàn)室中,。即將在八月出版的EMBO中,，Huimin Kong及其在New England Biolabs的同事描述了一種大量復(fù)制DNA的新方法，這種方法克服了之前的一些局限性,。
這項(xiàng)新技術(shù)稱作HAD（helicase-dependent amplification,，解鏈酶復(fù)制法）,。HAD和PCR一樣簡(jiǎn)單，但卻有明顯的優(yōu)勢(shì),。PCR要求熱循環(huán)系統(tǒng)來(lái)加熱和冷卻DNA樣品,，從而使其變性成單鏈、復(fù)制單鏈,、從而合成新的雙鏈DNA。而HAD則是模仿自然的DNA復(fù)制方法,，即利用一種解鏈酶使DNA變性,。因此，整個(gè)HAD反應(yīng)能夠在同一個(gè)溫度下進(jìn)行,，從而起到優(yōu)化合成并且不需要昂貴的,、耗電的熱循環(huán)器。
HAD能將DNA復(fù)制應(yīng)用到一些之前因PCR的特定要求而無(wú)法使用的情況,。這種復(fù)制方法的費(fèi)用相對(duì)比較低,，更重要的是由于HAD操作簡(jiǎn)便使它能用于開發(fā)便攜式DNA診斷儀器方面。這種便攜式診斷儀器可以用來(lái)定點(diǎn)檢測(cè)或在整個(gè)領(lǐng)域檢測(cè)病原物,。
原文：
Scientists have developed a new method for DNA amplification that could replace the polymerase chain reaction (PCR), a technique that is invaluable for both medical diagnostics and basic research but which is confined to the laboratory. In the August issue of EMBO reports, Huimin Kong and colleagues at New England Biolabs (Beverly, MA, USA) describe a way to copy mass amounts of DNA that overcomes some of the limitations of this earlier technique.
HDA (helicase-dependent amplification) is as simple as PCR, but has significant advantages. PCR requires thermocycling to heat and cool a sample of DNA, to allow denaturation (separating DNA into single strands) and synthesis (copying single strands to create new double-stranded DNA). HDA instead mimics nature's method of replicating DNA by using a helicase enzyme to denature the DNA. As a result, the entire HDA reaction can be performed at one temperature that is optimized for synthesis, eliminating the need for an expensive and power-hungry thermocycler.
HDA could expand the application of DNA amplification to situations in which the requirements for PCR have made it prohibitive. The costs are likely to be more modest and, most importantly, the simplicity of HDA makes it suitable for the development of hand-held DNA diagnostic devices that could be used to detect pathogens at the point-of-care or in the field.
