南開大學(xué)的科研人員在世界上首次完成了一株重要的采油微生物的全基因組破譯,，揭示了其遺傳信息并首次發(fā)現(xiàn)重要代謝路徑，為清除石油污染帶來新的曙光,，對于微生物采油技術(shù)的革新亦具有重要意義,。
日前，有關(guān)成果以論文的形式發(fā)表在國際頂級學(xué)術(shù)刊物《美國科學(xué)院院刊》（英文簡稱PNAS）上,。3月19日,，PNAS在其主頁上公布了這篇論文的全文。這是天津地區(qū)誕生的第一篇PNAS論文,。
論文通訊作者,、南開大學(xué)泰達(dá)生物技術(shù)學(xué)院院長、長江學(xué)者王磊是項(xiàng)目主持人,。他說,，該成果是石油微生物研究領(lǐng)域的重要發(fā)現(xiàn)，南開大學(xué)已對其中的關(guān)鍵發(fā)現(xiàn)申請國際專利保護(hù),。
部分英文原文：
Published online before print March 19, 2007, 10.1073/pnas.0609650104
PNAS | March 27, 2007 | vol. 104 | no. 13 | 5602-5607
Genome and proteome of long-chain alkane degrading Geobacillus thermodenitrificans NG80-2 isolated from a deep-subsurface oil reservoir
Lu Feng*,,, Wei Wang*,,, Jiansong Cheng*, Yi Ren*, Guang Zhao*, Chunxu Gao*, Yun Tang*, Xueqian Liu*, Weiqing Han*, Xia Peng*,,, Rulin Liu,, and Lei Wang*,,,,¶
*TEDA School of Biological Sciences and Biotechnology and Tianjin Key Laboratory of Microbial Functional Genomics, Nankai University, 23 Hongda Street, Tianjin Economic-Technological Development Area (TEDA), Tianjin 300457, China; Tianjin Research Center for Functional Genomics and Biochip, 23 Hongda Street, Tianjin Economic-Technological Development Area (TEDA), Tianjin 300457, China; and College of Life Sciences, Nankai University, 94 Weijin Road, Tianjin 300071, China
Edited by David M. Karl, University of Hawaii, Honolulu, HI, and approved February 6, 2007 (received for review November 2, 2006)
The complete genome sequence of Geobacillus thermodenitrificans NG80-2, a thermophilic bacillus isolated from a deep oil reservoir in Northern China, consists of a 3,550,319-bp chromosome and a 57,693-bp plasmid. The genome reveals that NG80-2 is well equipped for adaptation into a wide variety of environmental niches, including oil reservoirs, by possessing genes for utilization of a broad range of energy sources, genes encoding various transporters for efficient nutrient uptake and detoxification, and genes for a flexible respiration system including an aerobic branch comprising five terminal oxidases and an anaerobic branch comprising a complete denitrification pathway for quick response to dissolved oxygen fluctuation. The identification of a nitrous oxide reductase gene has not been previously described in Gram-positive bacteria. The proteome further reveals the presence of a long-chain alkane degradation pathway; and the function of the key enzyme in the pathway, the long-chain alkane monooxygenase LadA, is confirmed by in vivo and in vitro experiments. The thermophilic soluble monomeric LadA is an ideal candidate for treatment of environmental oil pollutions and biosynthesis of complex molecules.
adaptation | degradation | monooxygenase
Author contributions: L.F. and W.W. contributed equally to this work; L.F. and L.W. designed research; W.W., G.Z., C.G., Y.T., X.L., and W.H. performed research; L.F., W.W., J.C., Y.R., X.P., and R.L. contributed new reagents/analytic tools; L.F., W.W., J.C., Y.R., G.Z., and C.G. analyzed data; and L.F., W.W., J.C., and L.W. wrote the paper.
