FIGURE 1. Movement of mRNA on the ribosome
a, b, tRNA–mRNA interaction in the 70S initiation (a) and post-initiation (b) complexes. (E-tRNA was co-purified with the ribosome.) c, Electron-density difference map between the SD(U)9UUU(U)4 post-initiation and the SD(A)4AUG(A)9 initiation complexes. Positive differences are in grey; negative differences are in red. d, Movement of mRNA from initiation (yellow) to post-initiation (grey) gives rise to a simultaneous positional rearrangement and clockwise rotation of the SD duplex. e, f, Detailed view of the ribosomal environment of the SD duplex in the initiation (e) and post-initiation (f) complexes.
生物谷報道：轉(zhuǎn)位是核糖體功能的最重要特征之一，它對于一個基因的表達水平具有重要的影響,。而此前,，一直沒有發(fā)現(xiàn)這個過程中關(guān)于mRNA運動的結(jié)構(gòu)信息，即在翻譯的不同階段,，mRNA是怎樣和核糖體相互作用的呢,？
現(xiàn)在，Yusupova等科研人員描述了當(dāng)從初始狀態(tài)轉(zhuǎn)變?yōu)楹蟪跏紶顟B(tài)時在一個3'-5'方向上的mRNA轉(zhuǎn)動和運動,。這是對mRNA動態(tài)的首次實驗觀測,，它對了解轉(zhuǎn)位機制將具有廣泛的意義，該結(jié)果發(fā)表在最新的一期《Nature》雜志上,。
原文出處：
Nature Volume 444 Number 7117
Structural basis for messenger RNA movement on the ribosome
Gulnara Yusupova, Lasse Jenner, Bernard Rees, Dino Moras and Marat Yusupov
全文下載：
First paragraph | Full Text | PDF (861K) | Supplementary information
See also: Editor's summary
作者簡介：
YUSUPOVA Gulnara - Ingénieur de recherche
Office: ESBS
Personal Section: Link to the personal page:
http://www-bio3d-igbmc.u-strasbg.fr/~gula
About the Lab:
The Structural Biology and Genomics Laboratory (SBGL) is located near Strasbourg, in the north/east part of France (Alsace) close to the German border. The Lab. has been directed by Dr Dino Moras since its creation. The laboratory was created in 1980 and was originally named the "Laboratoire de Biologie Structurale" (Structural Biology Laboratory). It was then the fifth department of the IBMC (Institut de Biologie Moléculaire et Cellulaire), in downtown Strasbourg. The laboratory was aimed at the understanding of the molecular mechanism responsible for the fidelity of the aminoacylation reaction of transfer-RNA. More recently (1990), in a collaboration with the laboratory of Prof P. Chambon, the laboratory initiated a new project in the nuclear receptor (NR) field. At the end of 1994, we moved to the IGBMC (Institut de Génétique et de Biologie Moléculaire et Cellulaire). The laboratory's current focus is the study of the structure/function relationship of macromolecules, employing biophysical means to solve the 3D structure (Xray cristallography, Nuclear Magnetic Resonance and, in collaboration, Electron Microscopy). The scientific activities of the laboratory can be divided in four major themes :
Translation, with special emphasis on aminoacyl-tRNA synthetases and transfer RNA
DNA structure and Transcription. A major project of the laboratory is currently the structural investigation of nuclear receptors an their functional implications in activation and regulation of transcription. A new project has been started recently and concerns the structural investigation of the general transcription factor TFIIH
Proteins of therapeutical importance. Recently the structure of a membrane receptor has been elucidated FhuA (ferric hydroxamate uptake protein A), as well as a matrix metallo-protease
Genomics and bioinformatics plays a transversal role in the scientific activity of the laboratory and the laboratory has numerous ongoing projects in this field
Publications:
Pubmed
