生物谷報(bào)道：雖然一個(gè)多細(xì)胞生物內(nèi)的各種不同細(xì)胞類型包含同一套基因,，但它們卻具有很不相同的行為,。這些細(xì)胞狀態(tài)被認(rèn)為與染色質(zhì)狀態(tài)有關(guān)，也就是說(shuō),，與組蛋白和包
裹基因組的其他蛋白的修飾有關(guān)?，F(xiàn)在，單分子測(cè)序技術(shù)被用來(lái)構(gòu)建小鼠胚胎干細(xì)胞和其他兩種在發(fā)育上更先進(jìn)的細(xì)胞類型的染色質(zhì)狀態(tài)圖,，從而顯示了重要染色質(zhì)修飾在整個(gè)基因組范圍內(nèi)的分布,。這項(xiàng)研究成果發(fā)表在最新一期的《自然》雜志上。
該研究為將綜合染色質(zhì)甄別方法應(yīng)用于對(duì)各種不同的哺乳動(dòng)物細(xì)胞群,、包括癌癥等疾病中所出現(xiàn)的異常細(xì)胞發(fā)育情況進(jìn)行定性研究提供了一個(gè)框架,。
FIGURE 1. Comparison of ChIP-Seq and ChIP-chip data.
Direct comparison of H3K4me3 (green) and H3K27me3 (red) ChIP data across a 300-kb region in mouse ES cells from independent experiments assayed by SMS (absolute fragment counts) or tiling arrays (log P-values for enrichment relative to whole-cell extracts15).
原文出處：
Nature Volume 448 Number 7153
Genome-wide maps of chromatin state in pluripotent and lineage-committed cells p553
Tarjei S. Mikkelsen, Manching Ku, David B. Jaffe, Biju Issac, Erez Lieberman, Georgia Giannoukos, Pablo Alvarez, William Brockman, Tae-Kyung Kim, Richard P. Koche, William Lee, Eric Mendenhall, Aisling O'Donovan, Aviva Presser, Carsten Russ, Xiaohui Xie, Alexander Meissner, Marius Wernig, Rudolf Jaenisch, Chad Nusbaum, Eric S. Lander & Bradley E. Bernstein
doi:10.1038/nature06008
Abstract | Full Text | PDF (577K) | Supplementary information
See also: Editor's summary | News and Views by Baylin & Schuebel
作者簡(jiǎn)介：
Bradley E. Bernstein, M.D. Ph.D.
Assistant Professor of Pathology
Massachusetts General Hospital, Harvard Medical School, Boston, MA USA
