Figure 1. Arc/Arg3.1 Interacts with Endophilin and Dynamin
(A) Schematic diagram showing the domain structure of Arc/Arg3.1, endophilin 3, dynamin 2, and the yeast two-hybrid fragments of each.
(B) When coexpressed in HEK293 cells, Arc/Arg3.1 coIPs dynamin 2(503–871) but not dynamin 2(612–871) (lacks the PH domain). Control blots show expressed dynamin (dynamin Off) and immunoprecipitated Arc/Arg3.1 (Arc IP).
(C) Dynamin coIPs with Arc/Arg3.1 from a rat forebrain synaptosomal fraction (P2). Ten percent of lysate used for IP was routinely loaded in offered lane.
(D) Arc/Arg3.1 coIPs endophilin 2(172–368) and endophilin 3(172–347), but not endophilin 1(172–352) from HEK293 cells.
(E) Pan endophilin Ab detects coIP with Arc/Arg3.1 from rat forebrain P2 fraction. Samples were reblotted with Abs selective for individual endophilin A proteins. Arc/Arg3.1 IP fractions show enrichment for endophilin 2 and 3, but not endophilin 1.
生物谷報(bào)道：約翰·霍普金斯大學(xué)的研究人員最近發(fā)現(xiàn)一種特殊的蛋白質(zhì)可以促進(jìn)長(zhǎng)期記憶形成，幫助大腦記憶事情超過(guò)1~2小時(shí)。有兩篇文章發(fā)表在11月9日的《神經(jīng)元》上,，報(bào)告了這一研究結(jié)果。
該蛋白質(zhì)被稱為Arc,，它與記憶相關(guān)性行為（從鳥類的學(xué)習(xí)唱歌到嚙齒類動(dòng)物的3維空間意識(shí)）有關(guān)。對(duì)于人類,，Arc可能是某些具有隱匿性的在記憶相關(guān)性行為（諸如藥物成癮）有關(guān),。
過(guò)去，研究人員在老鼠實(shí)驗(yàn)中發(fā)現(xiàn)：在老鼠清醒和活躍的時(shí)候,，它們的大腦會(huì)產(chǎn)生大量的Arc蛋白,。盡管如此，Arc蛋白在細(xì)胞中的作用卻直到最近才被發(fā)現(xiàn),。
為了確切了解Arc蛋白的作用，約翰·霍普金斯大學(xué)的研究隊(duì)伍仔細(xì)尋找了哪些蛋白與Arc蛋白發(fā)生作用,。實(shí)驗(yàn)者使用Arc蛋白做“餌”（標(biāo)志物）,，在一大群正常情況下在大腦中存在的其他的蛋白質(zhì)中進(jìn)行“垂釣”，發(fā)現(xiàn)其與兩個(gè)已知的負(fù)責(zé)在細(xì)胞內(nèi)外轉(zhuǎn)運(yùn)物質(zhì)的蛋白質(zhì)可以相互作用,，即我們已知的發(fā)動(dòng)蛋白（dynamin ）和 endophilin,。因而證明了Arc通過(guò)控制著這兩個(gè)蛋白質(zhì)來(lái)控制著細(xì)胞吞噬其表面受體的頻率，并可能借此轉(zhuǎn)運(yùn)與記憶有關(guān)的物質(zhì),。這一發(fā)現(xiàn)這將有助于我們進(jìn)一步理解大腦是如何保存記憶的,。
文獻(xiàn)出處：
Neuron November 9, 2006: 52 (3)
"Arc, Synaptic Plasticity, and Memory"
[Cover Caption]
Arc/Arg3.1: Linking Gene Expression to Synaptic Plasticity and Memory
Anastassios V. Tzingounis and Roger A. Nicoll
[Summary] [Full Text] [PDF]
Arc/Arg3.1 Is Essential for the Consolidation of Synaptic Plasticity and Memories
Niels Plath et al
[Summary] [Full Text] [PDF] [Supplemental Data]
Arc/Arg3.1 Mediates Homeostatic Synaptic Scaling of AMPA Receptors,
Jason D. Shepherd et al.
SummaryPlus | Full Text + Links | PDF (2096 K)
