脊髓夾傷是一類臨床上常見的嚴重中樞神經(jīng)損傷，主要病因是外力導(dǎo)致的脊椎椎骨骨折和椎管內(nèi)腫瘤等。中國科學(xué)院遺傳與發(fā)育生物學(xué)研究所戴建武研究員領(lǐng)導(dǎo)的再生醫(yī)學(xué)實驗室與南京市鼓樓醫(yī)院神經(jīng)外科合作,，在脊髓夾傷后的神經(jīng)再生研究中取得了重要進展,。
脊髓夾傷后,，膠原蛋白在損傷部位大量表達,。戴建武實驗室研制出了能與膠原蛋白特異結(jié)合的基因工程重組人腦源性神經(jīng)營養(yǎng)因子（CBD-BDNF），提出了利用損傷部位的膠原蛋白作為結(jié)合靶點和再生支架的思路,。
他們在大鼠脊髓夾閉傷模型中證明,，脊髓夾傷部位的膠原蛋白可以作為CBD-BDNF的有效靶點。一次性鞘內(nèi)注射的CBD-BDNF能較長時間地存留在損傷部位進行治療,。CBD-BDNF能夠有效促進脊髓夾傷后神經(jīng)電位的傳導(dǎo),，神經(jīng)再生和運動能力的恢復(fù)。戴建武研究員表示,，該實驗是脊髓損傷修復(fù)研究長期目標(biāo)的又一次有效嘗試,，但這次更接近臨床情況。
這項工作以戴建武實驗室博士研究生韓倩倩和南京市鼓樓醫(yī)院神經(jīng)外科梁維幫為共同第一作者發(fā)表在Biomaterials雜志上,。
遺傳發(fā)育所戴建武研究員實驗室主要從事干細胞和再生醫(yī)學(xué)研究,，近年來在包括神經(jīng)再生和神經(jīng)損傷修復(fù)等多個領(lǐng)域取得了多項研究成果。（生物谷Bioon.com）
生物谷推薦原文出處：
Biomaterials doi:10.1016/j.biomaterials.2010.07.084
The promotion of neurological recovery in the rat spinal cord crushed injury model by collagen-binding BDNF
Weibang Lianga, 1, Qianqian Hanb, c, 1, Wei Jina, Zhifeng Xiaob, Jingchun Huangd, Hongbin Nia, Bing Chenb, Jie Konga, Jun Wua and Jianwu Daib, ,
a Department of Neurosurgery, The Affiliated Drum Tower Hospital, School of Medicine, Nanjing University, Nanjing 210008, China
b Key laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100080, China
c Graduate School, Chinese Academy of Sciences, Beijing 100080, China
d Shandong Quality Inspection Center For Medical Devices, China State Drug Administration (SFDA), JiNan 250101, China
Spinal cord crushed injury is clinically common. Promoting targeted neural regeneration at the crushed site of spinal cord could be important for the repair. It has been demonstrated in our previous work that native human BDNF fused with a collagen-binding domain (CBD-BDNF) can bind to collagen specifically to exert the neurotrophic effect on promoting axonal regeneration. After injury, collagen is highly accumulated at the injury site. We thus speculate that CBD-BDNF will bind to the extracellular matrix collagen and concentrate at the injury site to improve the therapy. Using the rat spinal cord crushed injury model, we have found that CBD-BDNF by one-time intrathecally injection could be retained and concentrated at the injury site for a longer time than native BDNF without collagen-binding domain. CBD-BDNF could promote better neural regeneration and locomotion recovery.
