外周神經(jīng)損傷會引起感覺機能和運動機能障礙，嚴重影響患者日后的生活質(zhì)量,。中國科學院遺傳與發(fā)育生物學研究所戴建武研究員領(lǐng)導(dǎo)的再生醫(yī)學實驗室與大連醫(yī)科大學腦疾病研究所合作,，在周圍神經(jīng)損傷后的再生研究中取得了重要進展。
損傷神經(jīng)的再生與功能的恢復(fù)依賴于局部的微環(huán)境,。研究表明,，給予軸突再生正確有序的引導(dǎo)，并提供具有神經(jīng)營養(yǎng)和保護功能的因子是促進再生關(guān)鍵,。戴建武實驗室研制了可引導(dǎo)并促進周圍神經(jīng)再生的有序功能生物支架材料,，將有序膠原材料用化學交聯(lián)的方法在表面包被對再生有促進作用層粘連蛋白,，并符合可特異與層粘連蛋白靶向結(jié)合的基因工程重組人睫狀神經(jīng)營養(yǎng)因子（LBD-CNTF），來引導(dǎo)受損神經(jīng)的再生,，并有效發(fā)揮神經(jīng)保護和促進再生的功能,。
他們與合作者制備了大鼠坐骨神經(jīng)橫斷模型，將這種有序功能生物材料填充到硅膠導(dǎo)管中連接神經(jīng)斷端,。結(jié)果表明,，這種功能材料可以引導(dǎo)神經(jīng)纖維的有序生長，阻止再生神經(jīng)纖維在管腔內(nèi)發(fā)生彌散,，明顯加強了軸突,，雪旺細胞的再生和神經(jīng)纖維的髓鞘化。有效地促進了神經(jīng)通路的重建與功能的恢復(fù),。
這項工作以戴建武實驗室博士研究生曹佳妮為第一作者發(fā)表在Biomaterials上,。該課題受到國家自然基金資助。（生物谷Bioon.com）
生物谷推薦原文出處：
Biomaterials DOI:10.1016/j.biomaterials.2011.02.020
The use of laminin modified linear ordered collagen scaffolds loaded with laminin-binding ciliary neurotrophic factor for sciatic nerve regeneration in rats
Jiani Cao,Changkai Sun,Hui Zhao,Zhifeng Xiao,Bing Chen,Jian Gao,Tiezheng Zheng,Wei Wu,Shuang Wu,Jingyu Wang,Jianwu Dai
Nerve conduit provides a promising strategy for nerve injury repair in the peripheral nervous system (PNS). However, simply bridging the transected nerve with an empty conduit is hard to satisfy functional recovery. The regenerated axons may disperse during regeneration in the empty lumen, limiting the functional recovery. Our previous work had reported that linear ordered collagen scaffold (LOCS) could be used as a nerve guidance material. Here we cross-linked LOCS fibers with laminin which was a major component of the extracellular matrix in nervous system. Ciliary neurotrophic factor (CNTF) plays a critical role in peripheral nerve regeneration. But the lack of efficient CNTF delivery approach limits its clinical applications. To retain CNTF on the scaffold, a laminin binding domain (LBD) was fused to the N-terminal of CNTF. Compared with NAT-CNTF, LBD-CNTF exhibited specific laminin-binding ability and comparable neurotrophic bioactivity. We combined LBD-CNTF with the laminin modified LOCS fibers to construct a double-functional bio-scaffold. The functional scaffold was filled in silicon conduit and tested in the rat sciatic nerve transection model. Results showed that this functional biomaterial could guide the axon growth, retain more CNTF on the scaffolds and enhance the nerve regeneration as well as functional recovery.
Keywords: LOCS fibers; Laminin; LBD-CNTF; Nerve transection repair
