中科院上海藥物所林東海課題組和昆明動物研究所賴仞課題組合作,，探索高原滇蛙裸露皮膚在長日照、強紫外線環(huán)境下抗紫外線和迅速清除自由基機制保持氧化還原平衡的分子機制,。經(jīng)過合作研究,，研究組從滇蛙中發(fā)現(xiàn)了一種新型的、可以快速清除氧化自由基,、具有強抗氧化功效的皮膚抗氧化多肽antioxidin-RL,。
科研人員用NMR 技術(shù)解析了antioxidin-RL的溶液結(jié)構(gòu)，探測了它與氧化自由基ABTS+的相互作用,，發(fā)現(xiàn)該多肽上兩個酪氨酸殘基Tyr6,Tyr12與自由基形成共價結(jié)合,，而半胱氨酸殘基Cys10起迅速清除自由基的作用,。為進(jìn)一步研究和開發(fā)利用抗氧化多肽antioxidin-RL在生物醫(yī)學(xué)、抗氧化保護(hù)以及化妝品研發(fā)等方面的價值提供了理論基礎(chǔ),。（生物谷Bioon.com）
生物谷推薦原始出處：
Free Radical Biology and Medicine doi:10.1016/j.freeradbiomed.2010.01.036
Frog skins keep redox homeostasis by antioxidant peptides with rapid radical scavenging ability
Cunbao Liua, b, 1, Jing Hongb, c, 1, Hailong Yanga, b, 1, Jing Wua, b, Dongying Maa, b, Dongsheng Lia, Donghai Linc, , and Ren Laia, d,
a Biotoxin Unit of the Key Laboratory of Animal Models and Human Disease Mechanisms, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, Yunnan, China
b Graduate School, Chinese Academy of Sciences, Beijing, China
c Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, China
d Key Laboratory of Microbiological Engineering of the Agricultural Environment, Ministry of Agriculture, Life Sciences College, Nanjing Agricultural University, Nanjing, Jiangsu, China
The question of how amphibians can protect themselves from reactive oxygen species when exposed to the sun in an oxygen-rich atmosphere is important and interesting, not only from an evolutionary viewpoint, but also as a primer for researchers interested in mammalian skin biology, in which such peptide systems for antioxidant defense are not well studied. The identification of an antioxidant peptide named antioxidin-RL from frog (Odorrana livida) skin in this report supports the idea that a peptide antioxidant system may be a widespread antioxidant strategy among amphibian skins. Its ability to eliminate most of the 2,2′-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) radical tested within 2 s, which is much faster than the commercial antioxidant factor butylated hydroxytoluene, suggests that it has a potentially large impact on redox homeostasis in amphibian skins. Cys10 is proven to be responsible for its rapid radical scavenging function and tyrosines take part in the binding of antioxidin-RL to radicals according to our nuclear magnetic resonance assay.
