7月23日，Nature Review Molecular Cell Biology雜志在線發(fā)表了中國科學(xué)院生物物理研究所劉光慧研究員同美國索爾科生物學(xué)研究所（The Salk institute for Biological Studies）研究人員合作的關(guān)于干細(xì)胞多能性與表觀遺傳調(diào)控的綜述文章,，題為Navigating the epigenetic landscape of pluripotent stem cells的文章,。
文章從DNA甲基化、組蛋白修飾,、核小體組裝,、染色體高級(jí)結(jié)構(gòu)、染色體同核膜相互作用等水平系統(tǒng)闡述了表觀遺傳調(diào)節(jié)多能干細(xì)胞“干性（stemness）”的分子機(jī)制,，并對(duì)其人類疾病相關(guān)性和該領(lǐng)域的發(fā)展趨勢(shì)進(jìn)行了探討和展望,。
表觀遺傳調(diào)控（尤其是細(xì)胞核內(nèi)染色體高級(jí)組織形式）是目前干細(xì)胞研究領(lǐng)域的前沿和熱點(diǎn)問題。劉光慧研究員曾最早利用兒童早衰癥（progeria）誘導(dǎo)型多能干細(xì)胞（iPSC）模型研究了核膜對(duì)染色體功能的調(diào)控（Liu et al. Nature 2011）,，并利用第三代腺病毒載體介導(dǎo)的大片段DNA同源重組技術(shù)靶向矯正了兒童早衰癥iPSC中存在的LMNA點(diǎn)突變,，從而逆轉(zhuǎn)了加速衰老的病理表型（Liu et al. Cell Stem Cell 2011）。（生物谷Bioon.com）
doi:10.1038/nrm3393
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Navigating the epigenetic landscape of pluripotent stem cells
Mo Li1,4, Guang-Hui Liu1,2,4 & Juan Carlos Izpisua Belmonte1,3
Pluripotent stem cells, which include embryonic stem cells and induced pluripotent stem cells, use a complex network of genetic and epigenetic pathways to maintain a delicate balance between self-renewal and multilineage differentiation. Recently developed high-throughput genomic tools greatly facilitate the study of epigenetic regulation in pluripotent stem cells. Increasing evidence suggests the existence of extensive crosstalk among epigenetic pathways that modify DNA, histones and nucleosomes. Novel methods of mapping higher-order chromatin structure and chromatin–nuclear matrix interactions also provide the first insight into the three-dimensional organization of the genome and a framework in which existing genomic data of epigenetic regulation can be integrated to discover new rules of gene regulation.
